Accuracy of quantification was evaluated without and with Type-II correction (using relative isotope abundance) as well as utilizing the first isotopic peak (M+1). Spike experiments with lipid species pairs of various lipid classes were analyzed by flow injection analysis-FTMS. In this work, we evaluated FTMS quantification accuracy of lipid species affected by Type-II overlap. High-resolution mass spectrometry, such as Fourier-transform mass spectrometry (FTMS), is capable of resolving Type-II overlap depending on mass resolving power. This overlap occurs in series of lipid species differing only by number of double bonds (DBs) mainly because of the natural abundance of 13C-atoms. Lipidomics data require consideration of ions with near-identical masses, which comprises among others the Type-II isotopic overlap. Authors: Regensburg University Hospital, European Molecular Biology Laboratory, Germany University of Southern Denmark, Denmark Abstract
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